Attenuation and Phenotypic Stability of Influenza B/Texas/l/84 Cold-Adapted Reassortant Virus: Studies in Hamsters and Chimpanzees
Identifieur interne : 002166 ( Main/Exploration ); précédent : 002165; suivant : 002167Attenuation and Phenotypic Stability of Influenza B/Texas/l/84 Cold-Adapted Reassortant Virus: Studies in Hamsters and Chimpanzees
Auteurs : Mark H. Snyder ; William T. London ; Hunein F. Maassab ; Brian R. Murphy [États-Unis]Source :
- Journal of Infectious Diseases [ 0022-1899 ] ; 1989.
Abstract
The temperature sensitivity, level of attenuation, and phenotypic stability of an influenza B/Texas/1/84 x B/Ann Arbor/1/66 cold-adapted (ca) reassortant virus that received the RNA segments that encode the hemagglutinin and neuraminidase surface glycoproteins from the B/Texas/84 wild-type virus and the remaining six RNA segments from the B/Ann Arbor/66 ca donor virus were evaluated. In comparison to wild-type virus, the ca reassortant was restricted in replication in the upper and lower respiratory tracts of hamsters and chimpanzees. This clearly demonstrated that the six transferable RNA segments of the B/Ann Arbor/66 ca donor virus specify the attenuation phenotype for animals with a 37°C core body temperature. In addition, the ca virus retained the temperature-sensitive (ts) phenotype after replication in hamsters and chimpanzees. To further evaluate its phenotypic stability, the ca reassortant virus was isolated after 6-15 d of replication in hamsters that were immunosuppressed with cyclophosphamide, and the isolates were tested for their temperature sensitivity in vitro and for their level of replication in immunocompetent hamsters. The isolated viruses retained their ts and attenuation phenotypes even after prolonged replication in vivo. Thus, the B/Ann Arbor/66 ca donor virus can transfer the desired properties of attenuation and phenotype stability to its reassortants. The findings support the continued evaluation of ca reassortant influenza B virus vaccines in humans, including fully susceptible children.
Url:
DOI: 10.1093/infdis/160.4.604
Affiliations:
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Snyder</name><affiliation><wicri:noCountry code="subField">Arbor</wicri:noCountry></affiliation></author><author><name sortKey="London, William T" sort="London, William T" uniqKey="London W" first="William T." last="London">William T. London</name><affiliation><wicri:noCountry code="subField">Arbor</wicri:noCountry></affiliation></author><author><name sortKey="Maassab, Hunein F" sort="Maassab, Hunein F" uniqKey="Maassab H" first="Hunein F." last="Maassab">Hunein F. Maassab</name><affiliation><wicri:noCountry code="subField">Arbor</wicri:noCountry></affiliation></author><author><name sortKey="Murphy, Brian R" sort="Murphy, Brian R" uniqKey="Murphy B" first="Brian R." last="Murphy">Brian R. Murphy</name><affiliation></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Please address requests for reprints to Dr. Brian R. Murphy, Laboratory of Infectious Diseases, Building 7, Room 106, Bethesda</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Infectious Diseases</title><title level="j" type="abbrev">J Infect Dis.</title><idno type="ISSN">0022-1899</idno><idno type="eISSN">1537-6613</idno><imprint><publisher>The University of Chicago Press</publisher><date when="1989-10">1989</date><biblScope unit="vol">160</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="604">604</biblScope><biblScope unit="page" to="610">610</biblScope></imprint><idno type="ISSN">0022-1899</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-1899</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">The temperature sensitivity, level of attenuation, and phenotypic stability of an influenza B/Texas/1/84 x B/Ann Arbor/1/66 cold-adapted (ca) reassortant virus that received the RNA segments that encode the hemagglutinin and neuraminidase surface glycoproteins from the B/Texas/84 wild-type virus and the remaining six RNA segments from the B/Ann Arbor/66 ca donor virus were evaluated. In comparison to wild-type virus, the ca reassortant was restricted in replication in the upper and lower respiratory tracts of hamsters and chimpanzees. This clearly demonstrated that the six transferable RNA segments of the B/Ann Arbor/66 ca donor virus specify the attenuation phenotype for animals with a 37°C core body temperature. In addition, the ca virus retained the temperature-sensitive (ts) phenotype after replication in hamsters and chimpanzees. To further evaluate its phenotypic stability, the ca reassortant virus was isolated after 6-15 d of replication in hamsters that were immunosuppressed with cyclophosphamide, and the isolates were tested for their temperature sensitivity in vitro and for their level of replication in immunocompetent hamsters. The isolated viruses retained their ts and attenuation phenotypes even after prolonged replication in vivo. Thus, the B/Ann Arbor/66 ca donor virus can transfer the desired properties of attenuation and phenotype stability to its reassortants. The findings support the continued evaluation of ca reassortant influenza B virus vaccines in humans, including fully susceptible children.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li></region></list><tree><noCountry><name sortKey="London, William T" sort="London, William T" uniqKey="London W" first="William T." last="London">William T. London</name><name sortKey="Maassab, Hunein F" sort="Maassab, Hunein F" uniqKey="Maassab H" first="Hunein F." last="Maassab">Hunein F. Maassab</name><name sortKey="Snyder, Mark H" sort="Snyder, Mark H" uniqKey="Snyder M" first="Mark H." last="Snyder">Mark H. Snyder</name></noCountry><country name="États-Unis"><region name="Maryland"><name sortKey="Murphy, Brian R" sort="Murphy, Brian R" uniqKey="Murphy B" first="Brian R." last="Murphy">Brian R. 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